THE SINGLE BEST STRATEGY TO USE FOR NR1-SITE

Applying this method, Kalbaugh et al. assigned affinity and efficacy roles to positions in the LBDs on the NR1 and NR2A NMDAR subunits. In situ mutation of residues in direct connection with bound ligand afflicted both of those efficacy and affinity, even though positions that stabilize the shut cleft conformation without having a immediate ligand